Answer
Check whether network fluctuation during the daily backup job is what's actually disrupting the database connection — in one confirmed case, this exact recurring same-time daily hang (including a related license-check error for a mobile/data-collection component) traced to network instability during the backup window interrupting the connection between the application and the database, not to anything about the recent cloning activity itself. The timing correlation with a scheduled backup job is the key clue to chase down, rather than assuming the clone operation introduced a lasting configuration problem.